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Related Concept Videos

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

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Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
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Regulation of Nuclear Protein Sorting01:45

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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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Nuclear Export01:42

Nuclear Export

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The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
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Nuclear Export of mRNA02:31

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

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Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
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Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

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Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
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Related Experiment Video

Updated: Oct 9, 2025

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
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Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

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Nuclear pore complexes feel the strain.

C Patrick Lusk1, Megan C King1

  • 1Department of Cell Biology, Yale School of Medicine, New Haven, CT 06519, USA.

Molecular Cell
|December 17, 2021
PubMed
Summary
This summary is machine-generated.

Nuclear envelope tension expands the nuclear pore complex transport channel. This tension also induces asymmetry in the pore membrane, impacting cellular transport mechanisms.

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Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
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Area of Science:

  • Cell Biology
  • Structural Biology
  • Biophysics

Background:

  • The nuclear pore complex (NPC) regulates transport between the nucleus and cytoplasm.
  • Understanding NPC structure and function in a native cellular context is crucial.

Purpose of the Study:

  • To determine the in situ structure of the human nuclear pore complex.
  • To investigate the impact of nuclear envelope tension on NPC structure and function.

Main Methods:

  • Cryo-electron tomography (cryo-ET) was used to visualize the human NPC within intact cells.
  • Advanced image processing techniques were applied to reconstruct the NPC structure.

Main Results:

  • The study determined the cryo-electron tomography structure of the human NPC captured in cellulo.
  • Nuclear envelope tension was found to expand the central transport channel of the NPC.
  • Tension was also observed to impose asymmetry on the NPC's membrane.

Conclusions:

  • Nuclear envelope tension is a significant factor influencing NPC structure.
  • The findings provide new insights into the dynamic regulation of nucleocytoplasmic transport.