Intermittent pH control strategy in sludge anaerobic fermentation: Higher short-chain fatty acids production, lower...

The pH control to promote short-chain fatty acids (SCFAs) production during anaerobic alkaline fermentation basically focused on constant pH control. In this study, a simple and consumption-reducing intermittent pH control strategy at moderate temperature (23 ± 2 °C) was investigated with adjusting pH to 10 when naturally reduced to 8. The intermittent pH control strategy could alleviate the inhibition of acid-producing bacteria by strong alkaline and high FA concentration. Meanwhile, microbial diversity promoted by 6% and 69% while the relative abundance of acid-producing bacteria increased by 36% and 61% compared to blank and constant pH fermenters. The relative genes abundance related to amino acid metabolism and fatty acid production were mostly promoted and led to enhanced SCFAs production. In the long-term fermenter, the intermittent pH control strategy could result in a 68% reduction in alkali consumption and a 37% increase in SCFAs production compared to that of the constant pH at 10.