Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

13.1K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
13.1K
Cascaded Op Amps01:16

Cascaded Op Amps

789
Operational amplifiers (op-amps) are versatile electronic components that can be interconnected in a cascade - one after another in a linear sequence. This cascading is possible due to their infinite input resistance and zero output resistance, allowing them to maintain their input-output relationships even when connected in series.
In a cascaded system, each op-amp is referred to as a stage. The output of one stage drives the input of the subsequent stage. As the input signal passes through...
789
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

343
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
343

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using hierarchical Bayesian modelling to assess shedder test suitability.

Forensic science international. Genetics·2026
Same author

Probabilistic searching of databases compared to binary CODIS eligibility: An improved methodology for maximizing investigative leads using short tandem repeat DNA profiles.

Forensic science international. Genetics·2026
Same author

deepNoC: A deep learning system to assign the number of contributors to a short tandem repeat DNA profile.

Forensic science international. Genetics·2026
Same author

A matter of age: Investigation of the ability to classify epithelial cells of different anatomical origin in aged samples using imaging flow cytometry.

Forensic science international·2026
Same author

Influences of oils on the persistence and recovery of DNA.

Journal of forensic sciences·2026
Same author

Microbial Ecological Signatures Predict Pathogen Emergence and Multidrug Resistance in Cystic Fibrosis Airways up to a Year in Advance.

medRxiv : the preprint server for health sciences·2026
Same journal

Comparison of key diagnostics for probabilistic interpretation of STR mixture data generated with length-based and MPS methodologies.

Forensic science international. Genetics·2026
Same journal

Likelihood Ratios Given Activity-Level Propositions for DNA Transfer Evidence: Theoretical Foundations of the HaloGen Framework (Part I).

Forensic science international. Genetics·2026
Same journal

Likelihood Ratios Given Activity-Level Propositions for DNA Transfer Evidence: Practical Implementation and Simulation Studies Using the HaloGen Engine (Part II).

Forensic science international. Genetics·2026
Same journal

Forensic evaluation of 101 identity-informative SNPs in a Filipino population using massively parallel sequencing.

Forensic science international. Genetics·2026
Same journal

Reprocessing of hair roots using conventional forensic DNA analysis following RapidHIT® ID processing.

Forensic science international. Genetics·2026
Same journal

Analysis of low-input and degraded forensic DNA trace samples with NGS using the MiSeq FGx platform.

Forensic science international. Genetics·2026
See all related articles

Related Experiment Video

Updated: Oct 9, 2025

Laser-induced Forward Transfer for Flip-chip Packaging of Single Dies
08:21

Laser-induced Forward Transfer for Flip-chip Packaging of Single Dies

Published on: March 20, 2015

12.6K

Exploring tapelifts as a method for dual workflow STR amplification.

Belinda Martin1, Duncan Taylor2, Adrian Linacre1

  • 1Biological Sciences Building, Flinders University, Bedford Park 5042, Australia; Biological Sciences Building, College of Science and Engineering, Flinders University, Bedford Park 5042, Australia.

Forensic Science International. Genetics
|December 17, 2021
PubMed
Summary
This summary is machine-generated.

Using tapelifts for touch DNA analysis allows for replicate direct PCR testing from a single sample. This method ensures reproducibility and meets accreditation guidelines for forensic casework, enhancing DNA analysis reliability.

Keywords:
Direct PCRForensic scienceSTR DNA profilingTechnical replicateTouch DNA

More Related Videos

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

12.9K
Construction and Characterization of a Novel Vocal Fold Bioreactor
11:11

Construction and Characterization of a Novel Vocal Fold Bioreactor

Published on: August 1, 2014

9.3K

Related Experiment Videos

Last Updated: Oct 9, 2025

Laser-induced Forward Transfer for Flip-chip Packaging of Single Dies
08:21

Laser-induced Forward Transfer for Flip-chip Packaging of Single Dies

Published on: March 20, 2015

12.6K
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

12.9K
Construction and Characterization of a Novel Vocal Fold Bioreactor
11:11

Construction and Characterization of a Novel Vocal Fold Bioreactor

Published on: August 1, 2014

9.3K

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Direct PCR is used for reference materials but not widely for touch DNA analysis from casework.
  • Concerns exist regarding increased sensitivity, complex mixtures, and lack of sample reproducibility with current methods.
  • Touch DNA analysis from exhibits is crucial for forensic investigations.

Purpose of the Study:

  • To propose and evaluate the use of tapelifts for replicate direct PCR analysis of touch DNA.
  • To assess the feasibility of using a single tapelift for both direct PCR and extraction-based workflows.
  • To ensure technical replicate concordance and meet accreditation guidelines for forensic DNA analysis.

Main Methods:

  • Touch DNA was deposited on various substrates by multiple donors.
  • Tapelifts were separated into portions for replicate direct PCR analysis using VeriFiler™ Plus.
  • Single tapelifts were also divided for simultaneous direct PCR and extraction-based PCR workflows.

Main Results:

  • Replicate direct PCRs from single tapelifts showed no statistical difference in allele calls, RFU, or likelihood ratios (LRs).
  • Sub-samples from the same tapelift demonstrated high similarity, supporting their use as technical replicates.
  • Dual workflow analysis showed no significant difference in allelic yield or donor comparison LRs between direct and extraction-based PCR.

Conclusions:

  • Tapelift sample division facilitates reproducible direct PCR analysis for touch DNA, meeting accreditation standards.
  • Utilizing a single tapelift for both direct and extraction-based workflows offers enhanced sensitivity and sample preservation.
  • This approach improves the reliability and efficiency of touch DNA analysis in forensic casework.