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Related Experiment Video

Updated: Oct 9, 2025

Analysis and Imaging of Osteocytes

Analysis and Imaging of Osteocytes

Published on: November 29, 2024

Computational framework for analyzing flow-induced strain on osteocyte as modulated by microenvironment.

Yoshitaka Kameo¹, Masahiro Ozasa², Taiji Adachi¹

¹Department of Biosystems Science, Institute for Frontier Life and Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan; Department of Micro Engineering, Graduate School of Engineering, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan; Department of Mammalian Regulatory Network, Graduate School of Biostudies, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.

Journal of the Mechanical Behavior of Biomedical Materials

|December 17, 2021

Summary

This summary is machine-generated.

Changes in the osteocyte microenvironment, like reduced pericellular matrix (PCM) density and increased canalicular curvature, enhance mechanical strain on osteocyte processes. This computational study provides insights into bone mechanosensing.

Keywords:

Canaliculus Fluid–structure interaction simulation Interstitial fluid flow Mechanosensing Osteocyte

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Published on: May 21, 2020

Area of Science:

Biomedical Engineering
Cellular Mechanobiology
Skeletal Biology

Background:

Osteocytes, embedded within bone matrix, are crucial mechanosensory cells regulating bone remodeling.
Interstitial fluid flow through the lacuno-canalicular network induces mechanical strain on osteocyte processes.
The osteocyte microenvironment, including pericellular matrix (PCM) and canalicular ultrastructure, significantly influences this strain.

Purpose of the Study:

To develop and utilize a novel computational framework to analyze fluid-structure interaction.
To investigate how alterations in the osteocyte microenvironment affect flow-induced strain on osteocyte processes.
To evaluate the spatial distribution of strain based on changes in PCM density and canalicular curvature.

Main Methods:

Development of a novel computational framework for fluid-structure interaction analysis.
Computer simulations to model interstitial fluid flow and its effects on osteocyte processes.
Evaluation of strain distribution under varying PCM densities and canalicular curvatures.

Main Results:

A decrease in PCM density enhances local flow-induced strain on the osteocyte process membrane.
An increase in canalicular curvature also significantly enhances local flow-induced strain.
These microenvironmental changes are associated with aging and bone disease.

Conclusions:

The developed computational framework effectively evaluates cell-specific mechanical stimuli in bone.
Reduced PCM density and increased canalicular curvature amplify mechanical strain on osteocytes.
This research deepens the understanding of osteocyte mechanobiology and their mechanical environment in living bone.