Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Protein Sorting and Transport01:45

Overview of Protein Sorting and Transport

16.6K
Eukaryotic cells have different membrane-bound organelles with distinct protein requirements. The process by which proteins are targeted to a specific organelle is called protein sorting.
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation.  In gated transport, folded...
16.6K
Lipids as Anchors01:32

Lipids as Anchors

6.4K
In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
6.4K
The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

3.6K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.6K
Signal Sequences and Sorting Receptors01:41

Signal Sequences and Sorting Receptors

9.9K
Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...
9.9K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

5.1K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
5.1K
Role of ER in the Secretory Pathway01:17

Role of ER in the Secretory Pathway

5.9K
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ER sensing of lipid metabolism drives PRA family-dependent regulation of COPII vesicle transport.

Nature communications·2026
Same author

Optical Control of Membrane Viscosity Modulates ER-to-Golgi Trafficking.

ACS central science·2025
Same author

Ceramide sorting into non-vesicular transport is independent of acyl chain length in budding yeast.

Biochemical and biophysical research communications·2024
Same author

Protein sorting upon exit from the endoplasmic reticulum dominates Golgi biogenesis in budding yeast.

FEBS letters·2024
Same author

Impact of sphingolipids on protein membrane trafficking.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2023
Same author

GPI anchors: Regulated as needed.

The Journal of cell biology·2023

Related Experiment Video

Updated: Oct 9, 2025

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

9.0K

A Role for Lipids in Protein Sorting?

Auxiliadora Aguilera-Romero1, Manuel Muñiz1

  • 1Dept. Cell Biology, University of Seville, Seville, 41012 Spain;,

Chimia
|December 18, 2021
PubMed
Summary
This summary is machine-generated.

Membrane lipids, not just proteins, play a crucial role in sorting proteins within the secretory pathway. This study in yeast reveals how lipid diversity and protein-lipid interactions guide cell surface protein export from the endoplasmic reticulum.

More Related Videos

Author Spotlight: Tackling Challenges in Synthetic Cell Engineering
10:56

Author Spotlight: Tackling Challenges in Synthetic Cell Engineering

Published on: April 12, 2024

1.3K
Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

12.6K

Related Experiment Videos

Last Updated: Oct 9, 2025

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

9.0K
Author Spotlight: Tackling Challenges in Synthetic Cell Engineering
10:56

Author Spotlight: Tackling Challenges in Synthetic Cell Engineering

Published on: April 12, 2024

1.3K
Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

12.6K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Eukaryotic cell membrane compartments rely on diverse lipids and proteins for identity and function.
  • The secretory pathway synthesizes and sorts proteins and lipids to endomembrane systems.
  • While protein involvement in sorting is established, the role of lipids remains under investigation.

Purpose of the Study:

  • To investigate the role of membrane lipids in protein sorting within the secretory pathway.
  • To explore how lipid diversity influences the differential export of lipid-linked cell surface proteins from the endoplasmic reticulum in yeast.
  • To highlight the significance of protein-lipid interactions in cellular sorting mechanisms.

Main Methods:

  • Investigated protein sorting mechanisms in yeast.
  • Focused on the differential export of specific lipid-linked cell surface proteins from the endoplasmic reticulum.
  • Employed an interdisciplinary approach combining expertise from various scientific groups.

Main Results:

  • Demonstrated that membrane lipids actively contribute to protein sorting in the secretory pathway.
  • Showcased the differential export of lipid-linked proteins based on lipid composition.
  • Underscored the importance of lipid diversity in ensuring correct protein delivery.

Conclusions:

  • Lipids are critical determinants in the sorting of proteins within the secretory pathway.
  • Protein-lipid interactions are essential for regulating protein localization and function.
  • This research provides new insights into the complex mechanisms governing membrane protein trafficking.