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Oxygen diffusion coefficient of cell membranes.

T Koyama, T Araiso, M Mochizuki

    Advances in Experimental Medicine and Biology
    |January 1, 1986
    PubMed
    Summary
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    This study measured oxygen diffusion in cell membranes using fluorescence depolarization. Results show significant variations in oxygen diffusion coefficients across species and organs, suggesting potential for higher values in vivo.

    Area of Science:

    • Biophysics
    • Cellular Physiology
    • Membrane Dynamics

    Background:

    • Understanding oxygen diffusion within biological membranes is crucial for cellular respiration and function.
    • Previous studies have not fully elucidated the species- and organ-specific variations in oxygen diffusion coefficients within cell membranes.

    Purpose of the Study:

    • To measure the microviscosity of erythrocyte membranes across different animal species and in bull frog lung cells and myocytes.
    • To estimate the diffusion coefficient of oxygen molecules (DO2) in these membranes.
    • To investigate the relationship between membrane microviscosity, wobbling diameter, and oxygen diffusion.

    Main Methods:

    • Utilized nanosecond fluorescence depolarization technique with 1,6-diphenyl-1,3,5-hexatriene (DPH) as a fluorescent probe.

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  • Measured microviscosity in erythrocyte membranes (sheep, human, rat, rabbit) and bull frog lung cells and myocytes.
  • Calculated the diffusion coefficient of oxygen molecules (DO2) and wobbling diameter (Wd) based on microviscosity measurements.

    • Main Results:

    • Significant interspecies differences in DO2 were observed in erythrocyte ghosts (0.84-1.92 x 10^-7 cm²/sec) and wobbling diameters (10.7-12.1 Å).
    • Intra-organ differences in DO2 were found in bull frogs (erythrocytes: 1.07, lung: 2.18, myocytes: 2.64 x 10^-7 cm²/sec) with corresponding Wd values (12.2-13.4 Å).
    • Comparison with ideal fluid states suggests actual DO2 in cell membranes might be up to ten times higher than measured values.

    Conclusions:

    • Membrane microviscosity and oxygen diffusion coefficients exhibit considerable variation across species and organs.
    • The findings highlight the complex interplay between membrane structure and oxygen transport at the cellular level.
    • Further research is needed to validate potentially higher in vivo oxygen diffusion coefficients in cell membranes.