Bi-exponential modelling of reconstitution kinetics in trained cyclists

The aim of this study was to investigate the individual $$ reconstitution kinetics of trained cyclists following repeated bouts of incremental ramp exercise, and to determine an optimal mathematical model to describe $$ reconstitution.

Ten trained cyclists (age 41 ± 10 years; mass 73.4 ± 9.9 kg; $$ 58.6 ± 7.1 mL kg min^-1) completed three incremental ramps (20 W min^-1) to the limit of tolerance with varying recovery durations (15-360 s) on 5-9 occasions. $$ reconstitution was measured following the first and second recovery periods against which mono-exponential and bi-exponential models were compared with adjusted R² and bias-corrected Akaike information criterion (AICc).

A bi-exponential model outperformed the mono-exponential model of $$ reconstitution (AICc 30.2 versus 72.2), fitting group mean data well (adjR² = 0.999) for the first recovery when optimised with parameters of fast component (FC) amplitude = 50.67%; slow component (SC) amplitude = 49.33%; time constant (τ)_FC = 21.5 s; τ_SC = 388 s. Following the second recovery, W' reconstitution reduced by 9.1 ± 7.3%, at 180 s and 8.2 ± 9.8% at 240 s resulting in an increase in the modelled τ_SC to 716 s with τ_FC unchanged. Individual bi-exponential models also fit well (adjR² = 0.978 ± 0.017) with large individual parameter variations (FC amplitude 47.7 ± 17.8%; first recovery: (τ)_FC = 22.0 ± 11.8 s; (τ)_SC = 377 ± 100 s; second recovery: (τ)_FC = 16.3.0 ± 6.6 s; (τ)_SC = 549 ± 226 s).

W' reconstitution kinetics were best described by a bi-exponential model consisting of distinct fast and slow phases. The amplitudes of the FC and SC remained unchanged with repeated bouts, with a slowing of W' reconstitution confined to an increase in the time constant of the slow component.