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Published on: February 11, 2022
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A pooled RT-PCR testing strategy for more efficient COVID-19 pandemic management
Akif Ayaz1, Asli Guner Ozturk Demir1, Gurkan Ozturk1
1Istanbul Medipol University, Istanbul, Turkey.
Summary
Pooled reverse transcription polymerase chain reaction (RT-PCR) testing is a practical strategy to increase COVID-19 screening capacity. This method maintains RT-PCR sensitivity and can be performed with a pool size of 20 to optimize results and cost.
Area of Science:
- Molecular Biology
- Virology
- Public Health
Background:
- Reverse transcription polymerase chain reaction (RT-PCR) is crucial for managing COVID-19.
- Emerging SARS-CoV-2 variants strain current RT-PCR screening capacity.
- Pooled RT-PCR testing offers a potential solution to expand testing capabilities.
Purpose of the Study:
- To evaluate the impact of pool size and sample mixture on RT-PCR cycle threshold (Ct) values.
- To determine the feasibility of pooled RT-PCR for COVID-19 screening.
- To assess if pooled testing affects the sensitivity of RT-PCR.
Main Methods:
- Investigated various mixtures of negative and positive COVID-19 samples.
- Tested pool sizes of 5, 10, and 20 samples.
- Analyzed the effect of low and high Ct positive samples within pools.
Main Results:
- Average baseline Ct and the number of high/low Ct samples significantly influenced final Ct values.
- Pool size did not show a significant association with final Ct values, though it was suggestive.
- Pooled RT-PCR testing did not reduce the sensitivity of the assay.
Conclusions:
- Pooled RT-PCR testing is a viable strategy to enhance COVID-19 screening capacity during a pandemic.
- A pool size of 20 is recommended for practical implementation, balancing efficiency and cost.
- This approach maintains RT-PCR sensitivity, making it a reliable method for increased testing.
