Elevation of NO₃^--N from biochar amendment facilitates mitigating paddy CH₄ emission stably over seven years

Biochar application into paddy is an improved strategy for addressing methane (CH₄) stimulation of straw biomass incorporation. Whereas, the differentiative patterns and mechanisms on CH₄ emission of straw biomass and biochar after long years still need to be disentangled. Considering economic feasibility, a seven-year of field experiment was conducted to explore the long-term CH₄ mitigation effect of annual low-rate biochar incorporation (RSC, 2.8 t ha^-1), with annual rice straw incorporation (RS, 8 t ha^-1) and control (CK, with no biochar or rice straw amendment incorporation) as a comparation. Results showed that RSC mitigated CH₄ emission while RS stimulated CH₄ significantly (p < 0.05) and stably over 7 experimental years compared with CK. RSC mitigated 14.8-46.7% of CH₄ emission compared with CK. In comparison to RSC, RS increased 111-950.5% of CH₄ emission during 7 field experimental years. On the 7th field experimental year, pH was significantly increased both in RS and RSC treatment (p < 0.05). RSC significantly (p < 0.05) increased soil nitrate (NO₃^--N) compared with RS while RS significantly (p < 0.05) increased dissolved carbon (DOC) compared to RSC. Soil NO₃^--N inhibition on methanogens and promotion on methanotrophs activities were verified by laboratory experiment, while soil pH and DOC mainly promoted methanogens abundance. Significantly (p < 0.05) increased DOC and soil pH enhanced methanogens growth and stimulated CH₄ emission in RS treatment. Higher soil NO₃^--N content in RSC than CK and RS contributed to CH₄ mitigation. Soil NO₃^--N and DOC were identified as the key factors differentiating CH₄ emission patterns of RS and RSC in 2019. Collectively, soil NO₃^--N impacts on CH₄ flux provide new ideas for prolonged effect of biochar amendment on CH₄ mitigation after years.