Development of a novel liquid crystal Apta-sensing platform using P-shape molecular switch

Described herein is a liquid crystal (LC)-based aptasensor via employing the reorientation of LC triggered by the conformational changes of a P-shaped DNA structure. The structure consists of a short linker sequence as an immobilizer probe with ability to hybridize with the central part of the intact aptamer (Apt) sequence and an Apt terminal-locker (ATL) strand with complementary segments of the Apt terminal fragments. Bindings of two arm segments of the Apt sequence with the ATL strand enforces it to form a P-shaped configuration on the sensing platform. The selective interaction between the Apt strand and OTA leads to the disassembly of the Apt-ATL hybrid, collapse of the P-shaped structure, and consequently, transition of the optical appearance of the aptasensor texture. Determination of Ochratoxin A (OTA) in foods is an urgent demand in attempt to minimize food safety risks. To demonstrate the feasibility of our aptasensing design, the OTA specific aptamer was selected as a model. The developed LC aptasensor possesses a wide linear range from 0.01 aM to 100 pM, ultra-low limit of detection (LOD) of 0.0078 aM, and quantitative recoveries of 91-103.51% for OTA in rice and grape juice samples. This study proposes a novel and universal LC-based platform for facile, ultra-sensitive, and precision sensing of hazardous analytes in real samples.