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Related Experiment Video

Updated: Oct 9, 2025

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

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Published on: July 21, 2017

Autophagy modulates cell fate decisions during lineage commitment.

Kulbhushan Sharma^1,2,3, Nagham T Asp^1,2, Sean Harrison⁴

¹Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.

|December 20, 2021

Summary

Autophagy

Keywords:

Autophagosome SOX2 differentiation ectoderm endoderm mesoderm pluripotent stem cells

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Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

Area of Science:

Stem cell biology
Developmental biology
Cellular processes

Background:

Pluripotency exit and germ layer commitment are critical developmental events.
Autophagy plays a known role in development and differentiation.

Purpose of the Study:

To investigate the role of autophagy in early lineage commitment.
To understand how autophagy influences pluripotency exit and germ layer specification.

Main Methods:

Utilized human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs).
Manipulated autophagy levels using chemical inhibitors and genetic approaches.
Assessed SOX2 protein levels and lineage markers.

Main Results:

A decrease in autophagy facilitates exit from pluripotency.
Modulating autophagy impacts SOX2 levels and lineage commitment.
Autophagy induction promotes SOX2 degradation and mesendoderm formation.
Autophagy inhibition leads to SOX2 accumulation and neuroectoderm formation.

Conclusions:

Autophagy-mediated SOX2 turnover is a key determinant of lineage commitment.
Understanding this mechanism can improve derivation of specific cell types.
This study provides insights into early developmental pathways.