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Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
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Arabidopsi s Spliceosome Factor SmD3 Modulates Immunity to Pseudomonas syringae Infection

Anna Golisz1, Michal Krzyszton1, Monika Stepien1

  • 1Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland.

Frontiers in Plant Science
|December 20, 2021
PubMed

Insights

SmD3 protein is crucial for plant immunity, affecting pre-mRNA splicing and stomatal development. Mutants show increased susceptibility to Pseudomonas syringae, indicating SmD3

Area of Science:

  • Plant molecular biology
  • Plant pathology
  • Plant immunity

Background:

  • SmD3 is a key component of small nuclear ribonucleoproteins (snRNPs), essential for pre-mRNA splicing.
  • The function of SmD3 in plant immunity is not well understood.

Purpose of the Study:

  • To investigate the role of Arabidopsis SmD3 in plant defense against Pseudomonas syringae pv. tomato (Pst) DC3000.
  • To determine the molecular mechanisms underlying SmD3's contribution to plant immunity.

Main Methods:

  • Analysis of smd3a and smd3b mutants' sensitivity to Pst DC3000 infection and its effectors (flg22, elf18, COR).
  • Genome-wide transcriptome analysis (RNA-seq) of smd3b-1 mutant infected with Pst.
  • Verification of gene expression changes using Northern blot and RT-qPCR.
  • Assessment of stomatal development and function.

Main Results:

  • smd3 mutants exhibit enhanced susceptibility to Pst infection and altered expression of pathogenesis markers.
  • SmD3-b deficiency deregulates plant defense at transcriptional and posttranscriptional levels, including splicing defects.
  • SmD3-b dysfunction primarily impairs stomatal immunity due to defects in stomatal development.
  • Mutants show altered responses to Pst effectors, including callose deposition and reactive oxygen species (ROS) production.

Conclusions:

  • SmD3 plays a significant role in Arabidopsis immunity, particularly in stomatal defense.
  • SmD3 regulates plant immune responses through the splicing of key pathogenesis-related factors.
  • Defects in stomatal development and function are a primary cause of altered mutant responses to pathogens.
Keywords:
PAMPSPst DC3000RNA-seqalternative splicingbiotic stressmiRNAplant immunity

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