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Circular RNA hsa_circ_0001658 Inhibits Intervertebral Disc Degeneration Development by Regulating

Ge-Dong Meng1,2, Bao-Shan Xu3

  • 1Tianjin Medical University, Tianjin 300070, China.

Computational and Mathematical Methods in Medicine
|December 20, 2021
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Circular RNA hsa_circ_0001658 is upregulated in intervertebral disc degeneration (IDD) and inhibits IDD by regulating hsa-miR-181c-5p and FAS. This finding offers a potential therapeutic target for IDD.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Intervertebral disc degeneration (IDD) is a debilitating condition with complex molecular underpinnings.
  • Non-coding RNAs (ncRNAs) play crucial roles in cellular processes and disease development.

Purpose of the Study:

  • To investigate the role of circular RNA hsa_circ_0001658 in IDD.
  • To elucidate the regulatory network involving hsa_circ_0001658, hsa-miR-181c-5p, and FAS in human neural progenitor cells (hNPCs).

Main Methods:

  • Microarray data analysis of nucleus pulposus (NP) tissues from IDD patients.
  • Bioinformatic analysis using starBase to predict circRNA-miRNA-mRNA interactions.
  • Experimental validation of the functional effects of hsa_circ_0001658 and hsa-miR-181c-5p on hNPC proliferation and apoptosis.

Main Results:

  • hsa_circ_0001658 was significantly upregulated, while hsa-miR-181c-5p was downregulated in IDD tissues.
  • Overexpression of hsa_circ_0001658 or silencing of hsa-miR-181c-5p inhibited hNPC proliferation and promoted apoptosis.
  • hsa_circ_0001658 functions as a molecular sponge for hsa-miR-181c-5p, which in turn regulates FAS expression.

Conclusions:

  • Circular RNA hsa_circ_0001658 plays a critical role in IDD pathogenesis by modulating the hsa-miR-181c-5p/FAS pathway.
  • hsa_circ_0001658 represents a promising therapeutic target for intervertebral disc degeneration.