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Updated: Oct 9, 2025
Measuring Retinal Vessel Diameter from Mouse Fluorescent Angiography Images
Published on: May 19, 2023
Quantitative analysis of retinal vasculature in normal eyes using ultra-widefield fluorescein angiography
Jing-Wen Jiang1, Zuo-Hui-Zi Yi1, Xiao-Ling Wang1
1Eye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
Aim:
To quantify the area and density of retinal vascularity by ultra-widefield fluorescein angiography (UWFA).
Methods:
In a retrospective study, UWFA images were obtained using an ultra-widefield imaging device in 42 normal eyes of 42 patients. Central and peripheral steered images were used to define the edge of retinal vasculature by a certified grader. The length from the center of the optic disc to the edge of retinal vascularity (RVL) in each quadrant and the total retinal vascular perfusion area (RVPA) were determined by the grader using OptosAdvance software. The density of retinal vascularity (RVD) was quantified in different zones of central-steered images using Image J software.
Results:
Among 42 healthy eyes, the values for mean RVL in each quadrant were 19.007±0.781 mm (superior), 18.467±0.869 mm (inferior), 17.738±0.622 mm (nasal) and 24.241±1.336 mm (temporal). The mean RVPA was 1140.117±73.825 mm2. The mean RVD of the total retina was 4.850%±0.638%. RVD varied significantly between different retina zones (P<0.001), and significant differences existed in the RVD values for total retinal area in patients over 50 years old compared to those under 50 years old (P=0.033). No gender difference was found.
Conclusion:
The UWFA device can be a promising tool for analyzing the overall retinal vasculature and may provide a better understanding of retinal vascular morphology in normal eyes. Aging may be related to lower RVD.
