[Detection of intracytoplasmic immunoglobulins by cytofluorography]

In this report the authors describe a method of cell permeabilisation compatible with flow cytometry. To maintain cell integrity, lysolecithin is used. This method is simple, rapid, and reproducible. Control tests demonstrated no non-specific fluorescence due to lysolecithin treatment. This technique was used in several cases of chronic lymphocytic leukemia to detect intracytoplasmic immunoglobulins and monoclonality and in one case of multiple myeloma.