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Related Experiment Video

Updated: Oct 9, 2025

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization
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Protocol for assessing senescence-associated lung pathologies in mice.

Koichiro Kawaguchi1, Michihiro Hashimoto2, Ryuta Mikawa1

  • 1Research Institute, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan.

STAR Protocols
|December 20, 2021
PubMed
Summary

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Eliminating senescent cells can alleviate lung aging and diseases like fibrosis and emphysema. This protocol uses ARF-DTR mice to study these senescence-dependent lung phenotypes.

Area of Science:

  • Gerontology
  • Pulmonology
  • Cell Biology

Background:

  • Cellular senescence is a key driver of tissue aging and associated pathologies, including lung diseases.
  • Eliminating senescent cells has shown promise in alleviating pulmonary conditions like fibrosis and emphysema in preclinical models.

Purpose of the Study:

  • To describe a protocol for assessing senescence-dependent lung phenotypes in mice.
  • To enable further research into the role of senescent cells in lung aging and pathology.

Main Methods:

  • Utilizes ARF-DTR mice for the semi-genetic elimination of senescent lung cells.
  • Incorporates pulmonary function testing to assess lung health.
  • Combines senescent cell elimination with established pulmonary disease models.
Keywords:
Cell BiologyHealth SciencesModel Organisms

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Main Results:

  • The protocol allows for the assessment of lung phenotypes influenced by cellular senescence.
  • Demonstrates a method to investigate the impact of senescent cell clearance on lung pathology.
  • Provides a framework for studying aging-associated lung diseases.

Conclusions:

  • The described protocol offers a valuable tool for studying the role of senescent cells in lung aging and disease.
  • Facilitates research into potential therapeutic strategies targeting senescent cells for pulmonary conditions.
  • Supports the investigation of senescence-dependent mechanisms in lung pathology.