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Multiscale 3D Bioprinting by Nozzle-Free Acoustic Droplet Ejection
Stefan Jentsch1, Ramin Nasehi1, Christoph Kuckelkorn1
1Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
Small Methods
|December 20, 2021
Summary
Acoustic droplet ejection (ADE) bioprinting offers a novel, nozzle-free method for creating complex 3D cell-laden hydrogels. This technique minimizes cell damage, preserving stem cell viability and function for tissue engineering applications.
Area of Science:
- Biotechnology
- Tissue Engineering
- Bioprinting
Background:
- Current bioprinting nozzle limitations include low resolution and cell damage due to shear stress.
- Nozzle clogging is a significant issue at resolutions below 100 µm.
Purpose of the Study:
- To introduce a novel 3D bioprinting method using acoustic droplet ejection (ADE).
- To overcome shear stress limitations and improve cell viability in bioprinted constructs.
Main Methods:
- Developed a 3D bioprinting technique based on acoustic droplet ejection (ADE).
- Utilized numerical simulations to compare shear stress with conventional microvalve nozzles.
- Demonstrated printing of cell clusters and single cells in hydrogels.
Main Results:
- The ADE method significantly minimizes critical shear stress compared to microvalve nozzles.
- Successfully printed cell-laden structures at both millimeter and single-cell scales.
- Confirmed no negative impact on stem cell morphology, proliferation, or differentiation.
Conclusions:
- Multiscale acoustic bioprinting is a cell-preserving technique for creating complex 3D hydrogel structures.
- This technology holds promise for individualized tissue engineering and regenerative medicine.
