A Nonenzymatic Hydrogen Peroxide Electrochemical Sensing and Application in Cancer Diagnosis

The diagnosis of malignant tumors is essential for informing clinical decisions regarding therapeutic options. Current imaging and pathological diagnostic methods do not provide quantitative molecular information that is important in tumor identification. Moreover, pathological tissue analysis is dependent on unevenly distributed pathological features. The tumor microenvironment has been documented to have hydrogen peroxide (H₂ O₂ ). This study presents a biologically sensitive and efficient H₂ O₂ electrochemical sensor based on PtNi nanoparticle-doped N-reduced graphene oxide (PtNi-N-rGO) with a low detection limit (2.8 × 10^-9 m), a fast response time (<6 s) and desirable anti-interference characteristics. Herein, H₂ O₂ is used as molecular biomarker. The sensor successfully captures H₂ O₂ from cancer cells. In addition, it efficiently detects tumor tissues, adjacent tissues, and normal tissues. This study demonstrates the H₂ O₂ sensor potential to rapidly detect tumor tissues. This technique provides a complementary method for pathological tumor diagnosis that is independent of the traditional pathology labs.