Microencapsulated Vitamin A Palmitate Degradation Mechanism Study To Improve the Product Stability

By using a high-resolution mass spectrometer, four vitamin A palmitate (VAP) degradants were identified from microencapsulated VAP degradation samples. Based on the degradants, VAP first breaks down into anhydroretinol (ANHR) and palmitic acid (PA) through ester thermal elimination (ETE). Sequentially, the formed ANHR reacts with remaining VAP to ANHR-VAP and with a second ANHR to ANHR-ANHR. The migration of H⁺ in the transition state predicts that the H⁺ concentration in media will affect the ETE. Based on the degradation mechanism discovered from this study, a new product was developed and its media pH changed from 4.2 to 6.2. The new microencapsulated VAP degraded from 22.3% to 4.8% on an annualized basis. In the VAP degradation, no oxidized apo-carotenoids were found. The oxidized apo-carotenoids were detected in the degradation of β-carotene, a pro-vitamin A, through natural oxidation by oxygen in air. This indicated that, in ambient and dry conditions on its own, VAP decay was unlike that of β-carotene through natural oxidation.