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RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
Published on: June 4, 2016
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Non-coding RNA regulates phage sensitivity in Listeria monocytogenes
Yuan Tian1,2, Liting Wu2, Mengya Zhu2,3
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
Plos One
|December 20, 2021
Summary
Deletion of the non-coding RNA rliB in Listeria monocytogenes impairs bacterial growth and invasion. This rliB defect also increases sensitivity to bacteriophage, offering potential for phage biocontrol strategies.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Non-coding RNAs (ncRNAs) play crucial roles in bacterial virulence and stress response.
- The CRISPR-family ncRNA rliB is conserved across Listeria species and its function is under investigation.
Purpose of the Study:
- To investigate the role of the ncRNA rliB in Listeria monocytogenes.
- To assess the impact of rliB deletion on bacterial growth, phage sensitivity, adhesion, invasion, and gene expression.
Main Methods:
- Homologous recombination was used to create rliB-defective mutants (Lm3-22-ΔrliB).
- Growth curves, phage sensitivity assays, adhesion and invasion assays, and quantitative real-time PCR were performed.
Main Results:
- Lm3-22-ΔrliB mutants exhibited slower growth and significantly increased sensitivity to Listeria phage vB-LmoM-SH3-3.
- Adhesion, invasion, and proliferation in host cells were significantly reduced in the mutants.
- Deletion of rliB altered the transcription of invasion-related genes, particularly in response to phage interaction.
Conclusions:
- The ncRNA rliB is a key regulator of Listeria monocytogenes virulence and host-pathogen interactions.
- rliB deletion weakens invasion and enhances phage susceptibility, suggesting potential for phage-based biocontrol of Listeria.
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