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Comprehensive analysis of the ceRNA network in coronary artery disease

Weikang Bian1, Xiao-Xin Jiang1, Zhicheng Wang1

  • 1Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006, Jiangsu, People's Republic of China.

Scientific Reports
|December 21, 2021
PubMed

Insights

This study identifies key regulatory networks in coronary artery disease (CAD) by analyzing gene expression. The findings reveal crucial long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) involved in CAD development.

Area of Science:

  • Genomics
  • Molecular Biology
  • Cardiovascular Research

Background:

  • Coronary artery disease (CAD) is a leading cause of mortality in aging populations.
  • The underlying genetic mechanisms of CAD remain incompletely understood.
  • Identifying genetic regulators is crucial for understanding CAD pathogenesis.

Purpose of the Study:

  • To construct a lncRNA-miRNA-mRNA regulatory network for CAD.
  • To systematically identify differentially expressed genes (DEGs) in CAD patients.
  • To elucidate the genetic basis of CAD development.

Main Methods:

  • Utilized lncRNA (GSE69587, GSE113079), miRNA (GSE105449), and mRNA (GSE113079, GSE9820) datasets from CAD and control samples.
  • Identified differentially expressed genes (DEGs) including 1111 lncRNAs, 2595 mRNAs, and 22 miRNAs.
  • Constructed a lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network to identify hub nodes.

Main Results:

  • A total of 1111 lncRNAs, 2595 mRNAs, and 22 miRNAs were identified as differentially expressed.
  • The ceRNA network highlighted KCNQ1OT1 and H19 lncRNAs with high connectivity to nine miRNAs.
  • Enriched pathways included nitrogen compound metabolism and PI3K-Akt signaling.

Conclusions:

  • The constructed ceRNA network provides insights into the molecular mechanisms of CAD.
  • Key lncRNAs like KCNQ1OT1 and H19 play significant roles in CAD pathogenesis.
  • Findings contribute to a better understanding of CAD development and potential therapeutic targets.

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