Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

229
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
229
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

47.5K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
47.5K
Retroviruses02:33

Retroviruses

12.8K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.8K
Subviral Agents01:29

Subviral Agents

177
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
177
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

205
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
205

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and Qualification of a Nipah Virus Glycoprotein-Specific IgG ELISA for the Assessment of Human Antibody Responses.

Vaccines·2026
Same author

Potent in vitro antiviral activity of 4'-Fluorouridine against diverse orthohantaviruses including Andes virus.

Antiviral research·2026
Same author

Targeted cleavage site mutations in the Gn precursor enable efficient generation of replication-competent rVSV-based surrogates for emerging nairoviruses.

Emerging microbes & infections·2026
Same author

Distinct respiratory and neurological disease phenotypes in lethal Nipah virus Malaysia versus Bangladesh infection in IFNAR knockout mice.

Antiviral research·2026
Same author

Nucleoprotein and glycoprotein based serological assays for detection of Marburg virus infections.

EBioMedicine·2026
Same author

Confirming ERVEBO Vaccination to Support Ebola Virus Surveillance.

Emerging infectious diseases·2026

Related Experiment Video

Updated: Oct 9, 2025

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.5K

A Pan-Respiratory Antiviral Chemotype Targeting a Host Multi-Protein Complex.

Maya Michon1, Andreas Müller-Schiffmann2, Anuradha F Lingappa1

  • 1Prosetta Biosciences, San Francisco, CA, USA.

Biorxiv : the Preprint Server for Biology
|December 21, 2021
PubMed
Summary

A novel antiviral compound targets host proteins involved in viral replication and immunity. This discovery offers a new therapeutic strategy for respiratory viral infections.

More Related Videos

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
11:34

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

14.0K
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

13.4K

Related Experiment Videos

Last Updated: Oct 9, 2025

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.5K
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
11:34

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

14.0K
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

13.4K

Area of Science:

  • Virology
  • Drug Discovery
  • Immunology

Background:

  • Respiratory viral infections pose a significant global health burden.
  • Existing antiviral therapies often face challenges with resistance and broad-spectrum efficacy.

Approach:

  • Developed a novel cell-free phenotypic screen to identify small molecule antivirals targeting viral capsid assembly.
  • Identified PAV-431, a compound effective against all six major human respiratory virus families in cell culture.
  • Demonstrated in vivo efficacy of the chemotype against Porcine Epidemic Diarrhea Virus and Respiratory Syncytial Virus.

Key Points:

  • PAV-431 binds to a specific subset of the 14-3-3 protein within a dynamic multiprotein complex.
  • This complex includes host proteins crucial for viral lifecycles and innate immunity.
  • Viral infection alters the complex composition, which PAV-431 treatment helps restore.

Conclusions:

  • PAV-431 represents a new antiviral chemotype targeting the host-virus interface.
  • This approach offers a novel therapeutic strategy for treating a broad range of respiratory viral diseases.
  • The findings suggest a new paradigm for antiviral drug development focused on host-pathogen interactions.