Dual-wavelength electrochemiluminescence ratiometry for hydrogen sulfide detection based on Cd²⁺-doped g-C₃N₄...

Herein, a novel and facile dual-wavelength ratiometric electrochemiluminescence-resonance energy transfer (ECL-RET) sensor for hydrogen sulfide (H₂S) detection was constructed based on the interaction between S^2- and Cd²⁺-doped g-C₃N₄ nanosheets (NSs). Cd²⁺-doped g-C₃N₄ NSs exhibited a strong ECL emission at 435 nm. In the presence of H₂S, CdS was formed in situ on g-C₃N₄ NSs by the adsorption of S^2- and Cd²⁺, generating another ECL emission at 515 nm. Furthermore, the overlapping of the absorption spectrum of the formed CdS and the ECL emission spectrum of g-C₃N₄ NSs led to a feasible RET, thus quenching the ECL intensity from g-C₃N₄ at 435 nm. Through an ECL decrease at 435 nm and an increase at 515 nm, a dual-wavelength ratiometric ECL-RET system for H₂S was designed. The sensor exhibited a lower detection limit of 0.02 μM with a wide linear range of 0.05-100.0 μM. In addition, the applicability of the method was validated by plasma sample analysis with a linear range of 80.0-106.0%. We believe that such a proposal would provide new insight into advanced dual-wavelength ECL ratiometric assays.