Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

16.2K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
16.2K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

4.6K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.6K
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

103
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
103
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.5K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.5K
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

3.4K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.4K
The Cell Cycle Control System01:28

The Cell Cycle Control System

3.8K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resilient nanostructured bioanalytic microneedle longitudinally monitors preclinical renal and hepatic drug clearance and dysfunction.

Science translational medicine·2026
Same author

Connecting genes to physiology: the first 25 years of <i>Physiological Genomics</i>.

Physiological genomics·2025
Same author

Transcription factor networks in cellular quiescence.

Nature cell biology·2025
Same author

Pan-cancer landscape of epigenetic factor expression predicts tumor outcome.

Communications biology·2023
Same author

Screen time: an unbiased search for histone mutations that affect quiescence and chronological aging.

The FEBS journal·2023
Same author

Soft strain-insensitive bioelectronics featuring brittle materials.

Science (New York, N.Y.)·2022

Related Experiment Video

Updated: Oct 9, 2025

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells
07:59

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Published on: October 4, 2013

8.6K

Stressed-out yeast do not pass GO.

Hilary A Coller1,2,3

  • 1Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA.

The Journal of Cell Biology
|December 21, 2021
PubMed
Summary
This summary is machine-generated.

Yeast cells respond to nutrient withdrawal by arresting cell division not only early, but also later in the cell cycle. The transcription factor Xbp1 is crucial for this later cell cycle arrest.

More Related Videos

Author Spotlight: Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons
07:59

Author Spotlight: Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

Published on: June 9, 2023

1.5K
Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
09:05

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Published on: April 18, 2016

29.3K

Related Experiment Videos

Last Updated: Oct 9, 2025

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells
07:59

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Published on: October 4, 2013

8.6K
Author Spotlight: Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons
07:59

Author Spotlight: Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

Published on: June 9, 2023

1.5K
Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
09:05

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Published on: April 18, 2016

29.3K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cell cycle progression is tightly regulated.
  • Nutrient availability is a key factor influencing cell division.
  • Endoplasmic reticulum stress can impact cellular processes.

Purpose of the Study:

  • To investigate the cell cycle response of individual yeast cells to nutrient withdrawal.
  • To identify molecular factors involved in cell cycle arrest during nutrient scarcity.

Main Methods:

  • Utilized microfluidics for precise control of cellular environment.
  • Employed advanced imaging techniques to monitor individual yeast cells.
  • Analyzed cell cycle progression and arrest points.

Main Results:

  • Observed cell cycle arrest not only in the early G1 phase but also at later stages.
  • Demonstrated that the transcription factor Xbp1 is essential for arrest at non-G1 phases.
  • Highlighted the role of endoplasmic reticulum stress in mediating cell cycle arrest.

Conclusions:

  • Nutrient withdrawal triggers complex cell cycle arrest mechanisms in yeast.
  • Xbp1-mediated endoplasmic reticulum stress response is critical for cell cycle regulation under nutrient limitation.
  • Findings provide new insights into cellular adaptation to environmental stress.