Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

5.6K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.6K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

893
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
893

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomics-based analysis of the defense mechanisms of disease-resistant grass carp against Aeromonas veronii.

Journal of proteomics·2026
Same author

Sweat and air permeable electronics enabled by engineered hierarchical fabric system for exercise management.

Microsystems & nanoengineering·2026
Same author

Strong interfacial electronic coupling activates NiFeOOH for alkaline seawater oxidation.

Chemical communications (Cambridge, England)·2026
Same author

[Short-term effectiveness of functional double-bundle anterior cruciate ligament reconstruction with internal brace by helical continuous suture fixation].

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery·2026
Same author

Characterization of proteoforms within α-synuclein complexes in living cells by cleavable cross-linking combined with top-down mass spectrometry.

Talanta·2026
Same author

Broadly tunable continuous-wave Tm:CALYO laser operating on the <sup>3</sup>H<sub>4</sub>→<sup>3</sup>H<sub>5</sub> transition.

Optics express·2026

Related Experiment Video

Updated: Oct 9, 2025

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
10:41

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

Published on: April 5, 2019

18.3K

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Hongwei Chu1,2, Qun Zhao1, Ju Liu3

  • 1CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023, China.

Analytical Chemistry
|December 21, 2021
PubMed
Summary

A novel ionic liquid (IL) extraction system, i-TAN, efficiently stabilizes hydrophobic membrane protein complexes (MPCs) and their interactions. This breakthrough enables deeper functional studies and reveals insights into drug resistance mechanisms.

More Related Videos

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

12.6K
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
09:54

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

10.9K

Related Experiment Videos

Last Updated: Oct 9, 2025

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
10:41

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

Published on: April 5, 2019

18.3K
Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

12.6K
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
09:54

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

10.9K

Area of Science:

  • Biochemistry and Molecular Biology
  • Membrane Protein Research
  • Drug Discovery and Development

Background:

  • Hydrophobic membrane protein complexes (MPCs) are crucial for cellular functions but difficult to study due to extraction challenges.
  • Existing extraction methods often disrupt protein-protein interactions (PPIs), hindering in-depth functional analysis of MPCs.
  • Efficient stabilization of MPCs during extraction is essential for understanding their biological roles and interactions.

Purpose of the Study:

  • To develop a novel, efficient extraction system for hydrophobic membrane protein complexes (MPCs).
  • To enhance the stability of protein-protein interactions (PPIs) during MPC extraction.
  • To investigate the potential of the new system in interactomics and understanding drug resistance mechanisms.

Main Methods:

  • Systematic screening of ionic liquids (ILs) for their ability to stabilize PPIs.
  • Development of a combined IL-detergent system (i-TAN) using triethylammonium acetate (TEAA) and Nonidet P-40 (NP-40).
  • Molecular docking simulations to confirm the stabilizing effect of TEAA.
  • Extraction and analysis of MPCs, including EGFR-interacting proteins, from breast cancer cell lines.

Main Results:

  • Triethylammonium acetate (TEAA) IL demonstrated significant potential in stabilizing PPIs.
  • The novel i-TAN extraction system (TEAA IL with 1% NP-40) showed superior performance in extracting and stabilizing MPCs.
  • i-TAN facilitated the identification of hydrophobic EGFR-interacting proteins and provided insights into drug resistance mechanisms by comparing sensitive and resistant cell lines.

Conclusions:

  • The i-TAN system offers a robust strategy for the extraction and stabilization of hydrophobic MPCs, overcoming limitations of previous methods.
  • This novel approach significantly advances the interactomics and functional analysis of MPCs.
  • The findings provide a foundation for understanding drug resistance mechanisms and developing new therapeutic strategies.