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Updated: Oct 9, 2025
A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy
Published on: May 16, 2015
Characterization of oxidative stress in animal model of neonatal hypoxia
Liliane de Mello Santos Bochenek1, Eduardo Benedetti Parisotto2, Evelyn de Andrade Salomão3
1Fellow Master degree. Postgraduate Program in Health and Development - Universidade Federal do Mato Grosso do Sul (UFMS) - Campo Grande (MS), Brazil.
Purpose:
To evaluate the oxidative stress in swine neonates submitted to hypoxia.
Methods:
Ten large white piglets, healthy newborns, of both sexes, were divided into two groups and submitted to an experimental hypoxia protocol with reduced inspired oxygen fraction. The hypoxia group, composed of six animals, was submitted to oxygen reduction for 180 min. The animals in the control group, n = 4, were handled and evaluated simultaneously, but without oxygen reduction.
Results:
180 min after the start of the hypoxic insult, a significant difference was observed in the oximetry, and heart rate of the hypoxia group was compared to the control group (p<0.05). There was no significant difference in the oxidative stress analyses. Reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (TBARS), protein carbonyl (PC), and myeloperoxidase (MPO) in the piglets' brain tissue were analyzed.
Conclusions:
Hypoxia causes adverse effects in swine neonates, although there is a natural physiological resistance of swine neonates to respond to this insult. Analyses of GSH, SOD, CAT, TBARS, MPO, and PC were tabulated and are presented as parameters for further studies to be carried out on an animal model of swine hypoxia.
