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Updated: Oct 9, 2025

In Vitro Reconstitution of Light-harvesting Complexes of Plants and Green Algae
Published on: October 10, 2014
Transforming Escherichia coli Proteomembranes into Artificial Chloroplasts Using Molecular Photocatalysis
Alexander K Mengele1, Dominik Weixler2, Sebastian Amthor1
1Institute of Inorganic Chemistry I, Materials and Catalysis, Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.
Abstract:
During the light-dependent reaction of photosynthesis, green plants couple photoinduced cascades of redox reactions with transmembrane proton translocations to generate reducing equivalents and chemical energy in the form of NADPH (nicotinamide adenine dinucleotide phosphate) and ATP (adenosine triphosphate), respectively. We mimic these basic processes by combining molecular ruthenium polypyridine-based photocatalysts and inverted vesicles derived from Escherichia coli. Upon irradiation with visible light, the interplay of photocatalytic nicotinamide reduction and enzymatic membrane-located respiration leads to the simultaneous formation of two biologically active cofactors, NADH (nicotinamide adenine dinucleotide) and ATP, respectively. This inorganic-biologic hybrid system thus emulates the cofactor delivering function of an active chloroplast.
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