MiR-194-3p modulates the progression of colorectal cancer by targeting KLK10
1Department of Gastrointestinal Surgery, Zhejiang Jinhua Guangfu Tumor Hospital, Jinhua, China.
Background:
A rich history of studies have manifested the importance of miRNAs to cancer progression, while miR-194-3p has been seldom explored.
Objective:
The purpose of this study is to unearth the way the KLK10/miR-194-3p axis modulates colorectal cancer (CRC).
Methods:
Differentially expressed genes of CRC in TCGA database were analyzed. Western blot and qRT-PCR were employed to test protein and mRNA expressions of two researched genes. Their targeting was confirmed using dual-luciferase. Biological behaviors of cells were tested by a series of cellular functional assays.
Result:
Remarkably low miR-194-3p expression and high KLK10 expression were observed in cancer cells. Overexpressing miR-194-3p hindered the progression of CRC cells. Overexpression of miR-194-3p significantly weakened the promoting effect of upregulated KLK10 on cell migration, invasion and proliferation. Their targeting was verified by dual -luciferase assay. Therefore, miR-194-3p hindered cell behaviors of CRC through KLK10.
Conclusion:
This investigation casts new light on the treatment of CRC through the KLK10/miR-194-3p axis.
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