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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

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Published on: November 13, 2021

An integrated device for fast and sensitive immunosuppressant detection.

Sara Tombelli¹, Cosimo Trono², Simone Berneschi¹

¹Institute of Applied Physics "Nello Carrara", CNR-IFAC, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy.

Analytical and Bioanalytical Chemistry

|December 22, 2021

Summary

This study introduces a novel optical device for point-of-care therapeutic drug monitoring. The compact system uses a microfluidic chip and fluorescent magnetic particles for rapid, sensitive detection of immunosuppressants.

Keywords:

Cyclosporine A Fluorescence Immunosuppressant Mycophenolic acid POCT

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Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Area of Science:

Biomedical Engineering
Analytical Chemistry
Clinical Diagnostics

Background:

Therapeutic Drug Monitoring (TDM) is crucial for optimizing immunosuppressant dosages.
Existing TDM methods can be time-consuming and require specialized laboratory settings.
There is a need for rapid, point-of-care (POC) diagnostic tools for TDM.

Purpose of the Study:

To develop and validate a compact optical device for POC TDM.
To enable simultaneous, sensitive detection of immunosuppressants.
To utilize microfluidics and advanced particle technology for improved assay performance.

Main Methods:

Design and integration of a disposable plastic microfluidic chip with ten parallel microchannels.
Incorporation of a microfluidic system for automated sample and reagent handling.
Utilization of submicrometric fluorescent magnetic particles for enhanced immunoassay efficiency.
Development of integrated thin-film amorphous silicon photodiodes for fluorescence detection.

Main Results:

Demonstration of a compact, integrated optical device for TDM.
Successful simultaneous detection of multiple analytes with replicate measurements.
Achieved enhanced assay sensitivity and lower limit of detection using fluorescent magnetic particles.
Validated the device's performance by detecting mycophenolic acid and cyclosporine A in spiked solutions and patient microdialysate samples.

Conclusions:

The developed optical POC device offers a promising solution for rapid TDM.
The use of microfluidics and fluorescent magnetic particles significantly improves assay speed and sensitivity.
This technology has the potential to enhance patient management through timely drug level monitoring.