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Hyperosmolarity Impairs Human Extravillous Trophoblast Differentiation by Caveolae Internalization
Julieta Reppetti1, Yollyseth Medina1, Mariana Farina2
1Laboratorio de Biología de la Reproducción, Instituto de Fisiología y Biofísica Bernardo Houssay (IFIBIO) - CONICET- Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
Frontiers in Physiology
|December 23, 2021
Summary
Hyperosmolarity impairs human extravillous trophoblast (EVT) cell differentiation by causing caveolae internalization and increasing their turnover. This impacts EVT cell migration, invasion, and tubulogenesis.
Area of Science:
- Cell Biology
- Reproductive Biology
- Biochemistry
Background:
- Caveolar structure is essential for human extravillous trophoblast (EVT) cell migration and tubulogenesis.
- Hyperosmolarity is known to induce caveolae internalization and accelerate their turnover.
- Signaling pathways regulating trophoblast differentiation are localized within caveolae.
Purpose of the Study:
- To investigate the hypothesis that hyperosmolarity impairs EVT differentiation.
- To determine the role of caveolae and caveolin-1 (Cav-1) in hyperosmolarity-induced effects on EVT cells.
Main Methods:
- Cultured human EVT cells (Swan 71 cell line) were exposed to a hyperosmolar condition (100 mM sucrose).
- Assessed effects on cell migration, tube-like structure formation, and cell invasion.
- Analyzed matrix metalloproteinase-2 (MMP-2) levels and caveolin-1 (Cav-1) protein abundance.
- Investigated the role of the lysosomal pathway and observed caveolae internalization via vacuole formation.
Main Results:
- Hyperosmolarity significantly altered EVT cell migration and tube-like structure formation.
- Cell invasion decreased, accompanied by reduced levels of active and latent matrix metalloproteinase-2 (MMP-2).
- Hyperosmolarity enhanced Cav-1 degradation via the lysosomal pathway, increased vacuole formation, and promoted caveolae internalization.
Conclusions:
- Hyperosmolarity compromises EVT cell differentiation.
- Caveolae internalization and accelerated turnover induced by hyperosmolarity play a critical role in impairing EVT cell function.
- Findings suggest a mechanism by which osmotic stress affects placental development.
Keywords:
caveolae/Cav-1cell migrationendovascular differentiationhuman extravillous trophoblasthyperosmolarityMore Related Videos
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