Alginate NiFe₂O₄ Nanoparticles Cryogel for Electrochemical Glucose Biosensor Development

Area of Science:

• Biomaterials Science
• Electrochemistry
• Nanotechnology

Background:

• Glucose biosensors are crucial for diabetes management and metabolic monitoring.
• Alginate cryogels offer a porous matrix for efficient enzyme immobilization.
• Enhancing electron transfer is key to improving biosensor sensitivity and performance.

Purpose of the Study:

• To develop a novel glucose biosensor using alginate cryogel.
• To incorporate NiFe2O4 nanoparticles for improved electrochemical detection.
• To optimize fabrication and operational parameters for enhanced glucose sensing.

Main Methods:

• Fabrication of alginate cryogel matrix.
• Immobilization of glucose-sensing enzymes within the cryogel.
• Incorporation of NiFe2O4 nanoparticles into the alginate matrix.
• Optimization of nanoparticle concentration and electrochemical detection conditions (scan rate, pH, buffer concentration).

Main Results:

• Optimized NiFe2O4 nanoparticle addition at 0.03 g/mL.
• Optimal electrochemical detection parameters identified: 0.11 V/s scan rate, pH 7.0, 150 mM buffer.
• Demonstrated a linear glucose response (R² = 0.98) with a regression line y = 18.18x + 455.28.
• Achieved a low detection limit of 0.32 mM and limit of quantification of 1.06 mM.

Conclusions:

• The developed alginate NiFe2O4 nanoparticles cryogel-based glucose biosensor is effective for electrochemical glucose determination.
• The incorporation of NiFe2O4 nanoparticles significantly enhances electron transfer and biosensor performance.
• Optimized parameters ensure reliable and sensitive glucose detection, showing potential for practical applications.