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B cell surface glycoprotein induced during growth response: molecular structure and expression pattern.

S O Southern, S L Swain, R W Dutton

    Journal of Immunology (Baltimore, Md. : 1950)
    |April 15, 1987
    PubMed
    Summary

    Researchers characterized the B 7.2 cell surface antigen, finding it expressed on activated B lymphocytes and in lymphoid tissues. This glycoprotein

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    Area of Science:

    • Immunology
    • Molecular Biology
    • Cell Biology

    Background:

    • B 7.2 is a cell surface antigen.
    • Its expression patterns and molecular characteristics are not fully understood.

    Purpose of the Study:

    • To perform molecular characterization of the B 7.2 antigen.
    • To investigate the expression patterns of B 7.2 in lymphoid tissues and B cells.

    Main Methods:

    • Analysis of B 7.2 expression in various B cell types and lymphoid tissues.
    • Biochemical characterization of the B 7.2 antigen's molecular structure.

    Main Results:

    • B 7.2 is expressed on activated B lymphocytes and constitutively on BCL1 and CH12 B lymphoma cells.
    • Expression is induced in resting B cells upon mitogenic stimulation and found frequently in fresh lymphoid tissues.

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  • Endogenous B 7.2 expression is high in spleen and lymph nodes, absent in the thymus.
  • B 7.2 is a microheterogeneous 45-50 kDa glycoprotein with a single polypeptide chain, intramolecular disulfide bonds, and N-linked glycans.
  • Its structure includes a cell-surface hydrophilic domain and a potential transmembrane hydrophobic segment.

    • Conclusions:

    • The B 7.2 antigen's expression pattern and molecular structure suggest a role in regulating B cell activation.
    • It may function as a receptor or ligand involved in B or T cell interactions.