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Updated: Oct 9, 2025
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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
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Identifying new molecular players in extracellular proteostasis
Sandeep Satapathy1,2, Mark R Wilson3,4
1Blavatnik Institute of Cell Biology, Harvard Medical School, Boston, MA 02115, U.S.A.
Biochemical Society Transactions
|December 23, 2021
Summary
Extracellular proteostasis, the balance of proteins outside cells, is poorly understood. This review explores known extracellular chaperones (ECs) and methods to find new ones, highlighting their multifunctional roles in tissue repair.
Area of Science:
- Cellular biology
- Biochemistry
- Physiology
Background:
- Proteostasis is crucial for cellular health, maintaining protein balance inside and outside cells.
- Understanding extracellular proteostasis is limited, with only 11 known extracellular chaperones (ECs).
Purpose of the Study:
- To review established extracellular chaperones (ECs).
- To discuss experimental strategies for identifying novel ECs.
- To examine putative new ECs and their functions.
Main Methods:
- Literature review of established ECs.
- Discussion of experimental approaches for EC identification.
- Analysis of newly identified putative ECs.
Main Results:
- All identified ECs are multifunctional proteins.
- ECs possess a unique combination of activities.
- ECs collectively act at sites of damage, infection, and inflammation.
Conclusions:
- Extracellular proteostasis is maintained by multifunctional ECs.
- ECs play a coordinated role in restoring homeostasis at damaged sites.
- Further research is needed to fully elucidate EC functions and interactions.
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