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Equine T lymphocytes express MHC class II antigens
Journal of Immunogenetics
|August 1, 1986
Summary
Researchers defined equine major histocompatibility complex (MHC) class II molecules using anti-HLA antibodies. These MHC class II antigens are expressed on resting horse lymphocytes, differing from humans but similar to dogs.
Area of Science:
- Immunology
- Veterinary Science
- Genetics
Background:
- Major histocompatibility complex (MHC) class II molecules play crucial roles in immune responses.
- Characterizing equine MHC class II is essential for understanding horse immunology.
Purpose of the Study:
- To define and characterize equine class II major histocompatibility complex (MHC) molecules.
- To investigate the expression patterns of MHC class II antigens on horse lymphocytes.
Main Methods:
- Utilized six anti-HLA class II mouse monoclonal antibodies (mAbs) and one rat anti-horse lymphocyte mAb.
- Employed ELISA, complement-dependent lymphocytotoxicity assays, and flow cytometry.
- Performed immunoprecipitation to analyze the molecular weight of the antigenic complex.
Main Results:
- Five anti-HLA class II mAbs and the anti-horse mAb reacted with peripheral blood lymphocytes.
- MHC class II antigens showed variable density on lymphocytes, including those lacking surface immunoglobulin.
- No reactivity was observed with equine platelets; immunoprecipitation revealed a 29,000-33,000 Mr complex.
Conclusions:
- Equine MHC class II distribution differs from humans but resembles dogs, with expression on resting lymphocytes lacking immunoglobulin.
- Findings provide insights into the functional properties of equine MHC class II molecules in immune responses.