Theoretical and Experimental Research on Multi-Layer Vessel-like Structure Printing Based on 3D Bio-Printing...

Area of Science:

• Biomedical Engineering
• Regenerative Medicine
• Materials Science

Background:

• Cardiovascular disease is a leading global cause of mortality.
• Limited donor availability hinders traditional autologous transplantation.
• Existing single-material printing lacks functional complexity for vessel regeneration.

Purpose of the Study:

• To investigate multi-material 3D bioprinting for multi-layer artificial vessel structures.
• To analyze the relationship between printing parameters and vessel formation.
• To validate the feasibility of bioprinting cell-loaded vessel constructs.

Main Methods:

• Theoretical analysis of vessel forming parameters (extrusion rate, rotating rod speed, platform speed).
• Experimental fabrication of vessel-like structures using sodium alginate and gelatin.
• 3D bioprinting of cell-loaded materials to assess viability.

Main Results:

• Synchronous relationships between extrusion rate, rotating speed, and platform speed were confirmed.
• Experimental parameters were corrected and validated using sodium alginate and gelatin.
• Printed constructs demonstrated over 90% cell viability.

Conclusions:

• Multi-material 3D bioprinting is a viable technique for fabricating multi-layer vessel-like structures.
• The study provides a validated theoretical framework and experimental parameters for artificial vessel development.
• This technology offers a promising solution for cardiovascular disease treatment by overcoming donor limitations.