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Updated: Oct 9, 2025

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
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Published on: May 27, 2022

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Molecular Variability and Host Distribution of 'Candidatus Phytoplasma solani' Strains from Different Geographic

Nicoletta Contaldo1, Jelena Stepanović2, Francesco Pacini1

  • 1Department of Agricultural and Food Sciences, Alma Mater Studiorum-University of Bologna, 40127 Bologna, Italy.

Microorganisms
|December 24, 2021
PubMed

Insights

Understanding phytoplasma genetic variability helps manage diseases. This study identified 25 molecular variants of Candidatus Phytoplasma solani, linking their distribution to geography and infection year for better disease monitoring.

Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Epidemiology

Background:

  • Phytoplasmas are plant pathogens causing significant agricultural damage.
  • Candidatus Phytoplasma solani (Ca. P. solani) is linked to grapevine bois noir and other plant diseases.
  • Understanding phytoplasma genetic diversity is key for disease epidemiology and management.

Purpose of the Study:

  • To analyze the genetic variability of Ca. P. solani strains.
  • To correlate genetic variations with geographic distribution and time of infection.
  • To provide insights for disease monitoring and forecasting.

Main Methods:

  • Restriction Fragment Length Polymorphism (RFLP) analyses.
  • Sequencing of vmp1, stamp, and tuf genes.
  • Examination of 119 Ca. P. solani strains from diverse hosts and regions.

Main Results:

  • Identification of 25 distinct molecular variants of Ca. P. solani.
  • Variability in vmp1, stamp, and tuf genes was associated with geographic origin and year of infection.
  • Differential strain distribution patterns were observed across regions, hosts, and time periods.

Conclusions:

  • Genetic variability of Ca. P. solani is geographically and temporally structured.
  • This molecular data aids in tracing epidemic cycles and understanding disease spread.
  • Findings support enhanced monitoring and forecasting strategies for Ca. P. solani-associated diseases.
Keywords:
ecologyepidemiologygenetic diversitymolecular characterizationphytoplasma disease

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