PCN-224 Nanoparticle/Polyacrylonitrile Nanofiber Membrane for Light-Driven Bacterial Inactivation

Area of Science:

• Materials Science
• Biotechnology
• Nanotechnology

Background:

• Rising antimicrobial resistance and pathogen spread necessitate innovative therapeutic strategies.
• Photodynamic antibacterial inactivation (aPDI) presents a promising approach to combat infectious diseases.

Purpose of the Study:

• To synthesize and evaluate a novel nanofiber membrane incorporating porphyrin-embedded metal-organic framework (MOF) material (PCN-224) for aPDI.
• To assess the antibacterial efficacy and biocompatibility of the developed PAN-PCN nanofiber membrane.

Main Methods:

• Synthesis of porphyrin-embedded MOF material (PCN-224).
• Embedding PCN-224 nanoparticles into polyacrylonitrile (PAN) nanofibers via electrospinning to create PAN-PCN nanofiber membranes.
• Antibacterial photodynamic inactivation (aPDI) assays against E. coli and S. aureus.
• Mechanism study focusing on singlet oxygen generation.
• MTT assay to evaluate biocompatibility.

Main Results:

• The PAN-PCN nanofiber membrane demonstrated significant bacterial elimination: 3.00 log units for E. coli and 4.70 log units for S. aureus under illumination.
• Singlet oxygen (1O2) was identified as the primary mechanism for bacterial inactivation.
• The nanofiber membranes exhibited good biocompatibility in the dark, with cell survival rates exceeding 85%.

Conclusions:

• The developed PAN-PCN nanofiber membrane effectively utilizes aPDI for bacterial inactivation.
• This material offers a stable, recyclable, and biocompatible platform for combating drug-resistant pathogens.
• The study highlights the potential of aPDI-enhanced nanofibers as a novel strategy against infectious diseases.