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Development of Silica Nanoparticle Supported Imprinted Polymers for Selective Lysozyme Recognition
Anika Kotyrba1, Mehmet Dinc2, Boris Mizaikoff1,2
1Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
Nanomaterials (Basel, Switzerland)
|December 24, 2021
Summary
Molecularly imprinted polymers (MIPs) were developed for selective hen egg white (HEW) lysozyme isolation. These synthetic materials demonstrate high binding capacity, aiding food analysis and quality control.
Area of Science:
- Materials Science
- Analytical Chemistry
- Biochemistry
Background:
- Protein imprinted materials, such as molecularly imprinted polymers (MIPs), offer significant potential in diverse analytical fields including clinical diagnostics, environmental monitoring, and drug delivery.
- Hen egg white (HEW) lysozyme, designated as food additive E 1105, is utilized for its preservative and antibacterial properties, necessitating reliable analytical methods for its detection and quantification.
- Accurate quality and regulatory control of food products containing HEW lysozyme requires efficient isolation techniques, which can be addressed by advanced molecularly imprinted materials.
Purpose of the Study:
- To synthesize and optimize imprinted core-shell particles as a synthetic recognition material specifically for hen egg white (HEW) lysozyme.
- To evaluate different synthesis routes and modifications for creating effective molecularly imprinted polymers (MIPs) for HEW lysozyme.
- To assess the performance of the developed MIPs in terms of binding capacity and selectivity for HEW lysozyme isolation.
Main Methods:
- Development of modified synthesis routes for creating imprinted core-shell particles.
- Utilizing hen egg white (HEW) lysozyme as the target analyte for imprinting.
- Characterization of the synthesized molecularly imprinted polymers (MIPs) for their recognition capabilities.
Main Results:
- The study successfully generated imprinted core-shell particles designed for selective HEW lysozyme recognition.
- The synthesized materials achieved a maximum binding capacity of 58.82 mg/g.
- Imprinting factors reached up to 2.74, indicating a high degree of selectivity for HEW lysozyme.
Conclusions:
- The developed molecularly imprinted polymers (MIPs) exhibit exceptional suitability for the selective isolation of HEW lysozyme.
- These materials provide a promising solution for quality and regulatory control analysis of HEW lysozyme in food matrices.
- The advancements in MIP synthesis contribute to the field of synthetic recognition materials for protein analysis.
