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Small Extracellular Vesicles Derived from Human Chorionic MSCs as Modern Perspective towards Cell-Free Therapy.

Jana Janockova1, Jana Matejova1, Marko Moravek1

  • 1Associated Tissue Bank, Faculty of Medicine, P. J. Safarik University and L. Pasteur University Hospital in Kosice, Tr. SNP 1, 04011 Kosice, Slovakia.

International Journal of Molecular Sciences
|December 24, 2021
PubMed
Summary

Researchers characterized human placental chorionic mesenchymal stem cells (CHo-MSCs) and their derived small extracellular vesicles (sEVs). These sEVs show potential for cell-free therapy due to their uptake by various cell types.

Keywords:
cell-free therapychorionextracellular vesiclesmesenchymal stem cellssmall EVs

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Regenerative Medicine

Background:

  • Mesenchymal stem cells (MSCs) are crucial for cell therapy, regenerative medicine, and tissue engineering.
  • Extracellular vesicles (EVs), particularly small EVs (sEVs)/exosomes from MSCs, are gaining research attention.
  • MSCs-derived exosomes offer a cell-free therapeutic strategy due to their safety and MSC-like properties.

Purpose of the Study:

  • To characterize MSCs from the human full-term placenta chorion (CHo-MSCs).
  • To isolate and analyze small EVs (sEVs) derived from these CHo-MSCs.
  • To investigate the uptake of these sEVs by target cells.

Main Methods:

  • Isolation and characterization of CHo-MSCs from human placenta chorion.
  • Isolation and analysis of small EVs (sEVs) from cultured CHo-MSCs.
  • Assessment of sEVs' uptake by synovial fibroblasts, osteoblasts, and periosteum-derived MSCs.

Main Results:

  • Successful isolation and characterization of CHo-MSCs.
  • Isolation and characterization of sEVs derived from CHo-MSCs.
  • Demonstrated uptake of CHo-MSC-derived sEVs by synovial fibroblasts, osteoblasts, and periosteum-derived MSCs.

Conclusions:

  • CHo-MSCs are a viable source for generating therapeutic sEVs.
  • Understanding MSC-derived sEVs' characteristics and mechanisms can advance therapeutic strategies.
  • CHo-MSC-derived sEVs show potential for cell-free therapeutic applications.