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Related Experiment Video

Updated: Oct 9, 2025

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
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Advances in Lipid Extraction Methods-A Review.

Ramesh Kumar Saini1, Parchuri Prasad2, Xiaomin Shang3

  • 1Department of Crop Science, Konkuk University, Seoul 143-701, Korea.

International Journal of Molecular Sciences
|December 24, 2021
PubMed
Summary

Efficient lipid extraction from biological tissues relies on careful solvent selection and cell disruption techniques. This review covers classical and modern methods, including green solvent approaches like supercritical CO2 extraction.

Keywords:
Bligh and Dyer methodFolch methodSoxhlet extractiongreen solventslipidomicspre-treatmentssupercritical CO2 extraction

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Lipidomics

Background:

  • Lipid extraction is fundamental for lipid analysis.
  • Solvent choice critically impacts extraction efficiency.
  • Cell wall disruption aids solvent penetration.

Purpose of the Study:

  • To review classical and modern lipid extraction methods.
  • To discuss environmentally friendly and rapid extraction techniques.
  • To cover high-throughput methods for lipidomics.

Main Methods:

  • Chloroform/methanol-based extraction.
  • Mechanical, chemical, and enzymatic cell disruption.
  • Supercritical CO2 extraction (SCE).

Main Results:

  • Classical methods and their modifications are detailed.
  • Green solvents and single-step extractions are highlighted.
  • High-throughput methods for LC-MS lipidomics are discussed.

Conclusions:

  • Optimizing solvent mixtures and pretreatment is key for efficient lipid recovery.
  • Modern methods focus on safety, speed, and environmental impact.
  • Emerging techniques like SCE offer promising alternatives.