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A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma
Yarong Yang1, Wenjuan Hua1, Mei Zeng1
1Department of Nuclear Medicine, Wuhan Fourth Hospital/Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
Open Medicine (Warsaw, Poland)
|December 24, 2021
Summary
Long noncoding RNA LINC00475 promotes papillary thyroid carcinoma (PTC) progression by increasing ZCCHC12 expression. This study elucidates the LINC00475/miR-376c-3p/ZCCHC12 pathway in PTC, offering potential therapeutic targets for this common thyroid cancer.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Papillary thyroid carcinoma (PTC) is the most common differentiated thyroid cancer.
- Long noncoding RNAs (lncRNAs) are implicated in various human cancers, including PTC.
Purpose of the Study:
- To investigate the function and mechanism of lncRNA LINC00475 in papillary thyroid carcinoma.
- To identify the molecular pathway involving LINC00475 in PTC progression.
Main Methods:
- Reverse-transcription polymerase chain reaction (RT-PCR) and subcellular fractionation to determine LINC00475 expression and location.
- Cell counting kit-8, ethynyl deoxyuridine (EdU) incorporation, wound healing, and Transwell assays to assess cell proliferation, viability, migration, and invasion.
- Luciferase reporter, RNA pulldown, and RNA immunoprecipitation (RIP) assays to confirm molecular interactions.
Main Results:
- LINC00475 was upregulated in PTC cells and localized in the cytoplasm.
- LINC00475 knockdown inhibited PTC cell viability, proliferation, migration, and invasion.
- LINC00475 upregulated ZCCHC12 expression by sponging miR-376c-3p, and ZCCHC12 overexpression partially rescued the inhibitory effects of LINC00475 knockdown.
Conclusions:
- LINC00475 promotes PTC cell proliferation, migration, and invasion.
- The mechanism involves LINC00475 upregulating ZCCHC12 via interaction with miR-376c-3p.
- LINC00475 acts as a potential oncogene in PTC progression.
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