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Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma
Published on: March 11, 2022
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Isolation and functional characterization of primary endothelial cells from mouse cerebral cortex
Julie Ouellette1,2, Baptiste Lacoste1,2,3
1Ottawa Hospital Research Institute, Neuroscience Program, Ottawa, ON K1H8M5, Canada.
STAR Protocols
|December 24, 2021
Summary
This study details a protocol for isolating and characterizing primary mouse brain endothelial cells (ECs). This method aids in assessing endothelial health and understanding EC roles in neurological disorders.
Area of Science:
- Neuroscience
- Cell Biology
- Vascular Biology
Background:
- Endothelial cells (ECs) are crucial for organ development, function, and maintenance.
- Brain ECs play a role in the onset and progression of neurological disorders.
- A need exists for reliable methods to study primary brain ECs.
Purpose of the Study:
- To present a detailed protocol for the isolation and characterization of primary mouse brain ECs.
- To enable assessment of endothelial health and function.
- To provide a tool for studying ECs in brain health and disease.
Main Methods:
- Detailed protocol for isolating primary mouse brain ECs.
- Inclusion of quality control measures.
- Incorporation of functional assays to assess EC viability and health.
Main Results:
- Successful isolation and characterization of primary mouse brain ECs.
- Procedures ensure EC survival for subsequent analysis.
- The protocol facilitates robust assessment of endothelial health.
Conclusions:
- The developed protocol is a valuable tool for neuroscience and vascular biology research.
- It enables better understanding of ECs in maintaining brain health.
- This method supports research into the role of ECs in neurological disorders.
