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Updated: Oct 8, 2025
Construction and Characterization of a Novel Vocal Fold Bioreactor
Published on: August 1, 2014
The Effects of Amniotic Membrane Transplantation on Vocal Fold Regeneration
Machiko Ogawa1,2, Shigeyuki Mukudai1, Yoichiro Sugiyama1
1Department of Otolaryngology Head and Neck Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Objectives/Hypothesis:
Vocal fold (VF) scar and sulcus cause severe vocal problems, but optimal methods have not been established. Total replacement of the mucosa is required particularly for cases in which the whole lamina propria is occupied by severe fibrosis and vibratory function is totally lost. The amniotic membrane (AM) has been proven to have regenerative potential, as it contains stem cells and growth factors. The current study investigated the biocompatibility and effects of AM for regeneration of the VF mucosa.
Study Design:
In vitro and in vivo studies.
Methods:
Vocal fold fibroblasts (VFFs) from 13 Sprague-Dawley rats were seeded on AM and subjected to histology and immunohistochemistry, and gene expressions in the VFFs on AM were examined in in vitro study. Twelve New Zealand White rabbits were used in in vivo study. VFs were stripped down and were reconstructed with AM. The regenerative effects were examined 3 months later by histological examination.
Results:
In vitro study indicated VFFs survived on AM and stained positively for Ki67, vimentin, and fibronectin. Gene expressions of Has1, Has2, and Hgf were significantly increased in the VFFs on AM compared with the other groups. The in vivo study indicated AM-transplanted VFs showed a significantly higher density of hyaluronic acid and lower density of collagen compared with sham VFs.
Conclusions:
The current preliminary study suggests biocompatibility and possible regenerative effects of AM for VFs.
Level Of Evidence:
NA Laryngoscope, 132:2017-2025, 2022.
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