Hydrogen sulfide as a potent scavenger of toxicant acrolein

Acrolein (ACR) is a metabolic byproduct in vivo and a ubiquitous environmental toxicant. It is implicated in the initiation and development of many diseases through multiple mechanisms, including the induction of oxidative stress. Currently, our understanding of the body defense mechanism against ACR toxicity is still limited. Given that hydrogen sulfide (H₂S) has strong antioxidative actions and it shares several properties of ACR scavenger glutathione (GSH), we, therefore, tested whether H₂S could be involved in ACR detoxification. Taking advantage of two cell lines that produced different levels of endogenous H₂S, we found that the severity of ACR toxicity was reversely correlated with H₂S-producing ability. In further support of the role of H₂S, supplementing cells with exogenous H₂S increased cell resistance to ACR, whereas inhibition of endogenous H₂S sensitized cells to ACR. In vivo experiments showed that inhibition of endogenous H₂S with CSE inhibitor markedly increased mouse susceptibility to the toxicity of cyclophosphamide and ACR, as evidenced by the increased mortality and worsened organ injury. Further analysis revealed that H₂S directly reacted with ACR. It promoted ACR clearance and prevented ACR-initiated protein carbonylation. Collectively, this study characterized H₂S as a presently unrecognized endogenous scavenger of ACR and suggested that H₂S can be exploited to prevent and treat ACR-associated diseases.