Aminoquinoline-anchored polynorbornene for sequential fluorescent sensing of Zn²⁺ and ATP

A novel aminoquinoline functionalized norbornene (1) and its ring-opening metathesis polymerization (ROMP) copolymer P1 have been designed and synthesized. The polymer probe P1 can self-assemble nano aggregation in aqueous solution. The fluorescent experiments revealed that both 1 and P1 show a ratiometric fluorescence response toward Zn²⁺ over other mental ions in Tris-HCl buffer solution, with the polymer probe P1 shows a better photostability and higher binding affinity than that of the small molecular probe 1. Furthermore, the in situ formed P1-Zn²⁺ ensemble was successfully used as the secondary sensor for ATP. P1 is also successfully used for monitoring intracellular Zn²⁺ and ATP in living cells.