[Bmim]FeCl₄ mediated inhibition and toxicity during anaerobic digestion: Dose-response kinetics, biochar-dependent...

[Bmim]FeCl₄, or 1‑butyl‑3-methylimidazolium tetrachloroferrate, is a typical ionic liquid (IL). Its recyclable, magnetic, multicomponent, and solvent-free nature makes it a particularly attractive ionic liquid for use in industrial processes. Despite its widespread use, the potential hazards that [Bmim]FeCl₄ might pose to the environment, including productive microorganisms, have not been explored. In this study, the dose-response of [Bmim]FeCl₄ in anaerobic digestion (AD) was investigated to assess the potential toxification and biochar-dependent detoxification in microbial communities, including enzymatic activity and molecule docking dynamics. Our results showed that methane production (31.52 mL_max/gVS) was sharply inhibited following [Bmim]FeCl₄ treatment. Moreover, increasing the dosage of [Bmim]FeCl₄ caused more dissolved organic matter (DOM) to be generated. Interestingly, 0.4 g/L of [Bmim]FeCl₄ could stimulate the high activity of microbial hydrolase and ATPase. However, a higher concentration of 2.65 g/L prevented these enzymatic processes from continuing. At the cellular level, higher concentration of [Bmim]FeCl₄ (>0.4 g/L) increased malondialdehyde (MDA) levels, leading to a higher cell lethal rate and weakening of the secondary structures of protein (especially, the amide I region). At the molecular level, the competitive H-bonding in the active sites caused low activity and consummated more energy. At the community level, structural equation modeling (SEM) revealed that [Bmim]FeCl₄ and biochar were the main drivers for microbial community succession. For instance, high [Bmim]FeCl₄ (8 g/L) benefited the growth of Clostridium sensu_stricto (from ≤1% to 27%). It is worth mentioning that biochar reversed the inhibition with high α-diversity, which caused a resurgence in the activity of previously inhibited ATPase and hydrolase. H₂-trophic methanogens (Methanolinea and Methaofastidisoum) were sensitive to [Bmim]FeCl₄ and decreased linearly while acetoclastic methanogens (Methanosaeta) were unchanged. These findings were consistent with the short-term activity tests and further verified by functional analysis.