Classification of breast cancer by a gold nanoparticle based multicolor fluorescent aptasensor

Breast cancer has seriously threatened women health in the world. Breast cancer classification may provide accurate molecular diagnosis information of the disease and prediction of tumor behavior to facilitate oncologic decision making. Here, we designed a dual-aptamers functionalized gold nanoprobe (DA-GNP) for classification of breast cancer based on Förster resonance energy transfer (FRET). The fluorescent labelled ER and HER2 (typical biomarker for breast cancer classification) specific aptamers are attached to gold nanoparticles' (GNPs) surface and fluorescence is quenched ultimately. The breast cancer subtype specific fluorescence will be recovered while the fluorescent labelled aptamer is bound to the biomarker protein, which are potentially useful for quantitative classification of different subtypes of breast cancer.