Bioaugmentation with a propionate-degrading methanogenic culture to improve methane production from chicken manure

Volatile fatty acid (VFA) accumulation caused by high ammonia concentrations is often encountered during the anaerobic digestion (AD) of ammonia-rich substrates. In this study, propionate-degrading methanogenic cultures were introduced to augment the semi-continuous AD of chicken manure under high ammonia levels. Introduction of a methanogenic culture enhanced the methane yield in the bioaugmented digester by 17-26% when the organic loading rate (OLR) was 2-4 g L^-1d^-1 compared to that in the control. When the OLR was further increased from 4.0 L^-1d^-1 to 5.0 g L^-1d^-1, and bioaugmentation ceased, methane yield improved by 15-18% under a high total ammonia nitrogen level of 5.0-8.4 g NH₄⁺-N/L. Moreover, bioaugmentation reconstructed the methanogenic community in the digester, promoting the dominance of hydrogenotrophic Methanobacterium and slightly increasing the abundance of aceticlastic Methanothrix and the syntrophic propionate-oxidizing bacteria Syntrophobacter, which were the key contributors to the improved AD under high ammonia concentrations.