Related Experiment Video
Updated: Oct 8, 2025
12:29
mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
9.3K
Plant RNA-mediated gene regulatory network
Behzad Hajieghrari1, Naser Farrokhi2
1Department of Agricultural Biotechnology, College of Agriculture, Jahrom University, Jahrom, Iran.
Genomics
|December 26, 2021
Summary
This review summarizes RNA-mediated gene silencing. Non-coding RNAs (ncRNAs) play crucial roles in regulating gene expression through sequence-specific mechanisms.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Gene expression relies on intricate regulatory networks.
- Not all transcribed RNAs code for proteins; some are non-coding RNAs (ncRNAs).
- ncRNAs are increasingly recognized for their significant roles in biological processes.
Purpose of the Study:
- To review the current understanding of RNA-mediated gene silencing.
- To highlight the importance of ncRNAs in gene regulation.
- To provide an overview of the complexity of RNA-mediated gene silencing.
Main Methods:
- Literature review of scientific publications.
- Synthesis of current knowledge on ncRNAs and gene silencing.
- Analysis of sequence-specific regulatory mechanisms.
Main Results:
- ncRNAs regulate gene expression at both transcriptional and post-transcriptional levels.
- RNA-mediated gene silencing is an ancient and complex biological machinery.
- Diverse ncRNAs are key players in gene regulatory pathways.
Conclusions:
- RNA-mediated gene silencing is a fundamental biological process.
- ncRNAs are essential regulators of gene expression.
- Further research into ncRNA function is crucial for understanding gene regulation.
Related Concept Videos
01:25Cell Signaling in Plants
5.8K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.8K
01:56Riboswitches
8.8K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.8K
01:23Regulation of Expression at Multiple Steps
1.1K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.1K
02:15Experimental RNAi
6.4K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.4K
02:24Regulation of Expression Occurs at Multiple Steps
23.8K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
23.8K
01:28Global Regulatory Systems
107
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
107