The effect of soil amendment derived from P-enhanced sludge pyrochar on ryegrass growth and soil microbial diversity

The application of pyrolyzed sewage sludge for land remediation is increasingly being considered as a technical solution to reuse nutrients in the sludge and mitigate the burden of sludge treatment. In this study, the enhancement effect of Ca-based additives, via phosphorus pyrolysis transformation promotion, was systematically investigated for the growth of ryegrass and soil microbial diversity. In the pot experiment, pyrochar-modified methods mainly changed the content of available phosphorus and organic matter in the soil and then affected ryegrass growth. Soils treated with pyrochar prepared with CaO and Ca(OH)₂ addition were dominated by phosphorus precipitation-capable Ramlibacter, while metal uptake-accelerating Massilia showed a high prevalence in the group treated with pristine sludge pyrochar. The results showed that the species composition of CaO and Ca(OH)₂ treated groups were similar, while the groups treated with Ca₃(PO₄)₂ and pristine sludge pyrochar exhibited similar compositional structures of microbial species. Furthermore, less than 3% of Pb accumulated in the shoots of the Ca-based additive-treated groups, but more than 35% of Pb was distributed in shoots treated with pristine sludge pyrochar. Therefore, the application of P-enhanced pyrochar adjusted by Ca-based additives to soil was beneficial to the growth of ryegrass and preventing metal transfer from soil to ryegrass. Based on both macroscopic and microscopic information, we summarized the promotion effect of P-enhanced pyrochar on ryegrass growth and soil physicochemical properties with the aim of designing a smart pyrochar for waste-to-resource applications.